Serum levels and urine detection of Centruroides sculpturatus venom in significantly envenomated patients.

TitleSerum levels and urine detection of Centruroides sculpturatus venom in significantly envenomated patients.
Publication TypeJournal Article
Year of Publication2009
AuthorsChase P, Boyer-Hassen L, McNally J, Vazquez HL, Theodorou AA, Walter FGardner, Alagón A
JournalClin Toxicol (Phila)
Volume47
Issue1
Pagination24-8
Date Published2009 Jan
ISSN Number1556-9519
KeywordsAged, 80 and over, Animals, Bites and Stings, Blotting, Western, Child, Preschool, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Inactivation, Metabolic, Infant, Scorpion Venoms, Scorpions, Severity of Illness Index, Time Factors
Abstract

<p><b>INTRODUCTION: </b>Envenomation by Centruroides sculpturatus can cause systemic signs and symptoms requiring treatment. The toxicokinetics of C. sculpturatus venom has not been described.</p>

<p><b>METHODS: </b>Venom components were separated for cross-reactivity testing. Serum and urine collected from three patients envenomated by C. sculpturatus had venom levels determined by sandwich enzyme-linked immunosorbent assay (ELISA).</p>

<p><b>RESULTS: </b>Western blot analysis indicated recognition of C. sculpturatus venom by Alacramyn, an equine F(ab&#39;)(2) antivenom developed against Centruroides scorpion venoms, including C. sculpturatus. Serum venom levels in ng/mL with post-envenomation times in minutes (min) were as follows: 85-year-old woman = 8.2 (approximately 150), 2.8 (515), 1.6 (1,200); 14-month-old girl = 29.7 (approximately 50), 5.0 (729); 3-year-old girl = 11.1 (approximately 313), urine venom level of 9.0 (approximately 490).</p>

<p><b>CONCLUSION: </b>There is sufficient venom cross-antigenicity among different Centruroides species to allow this ELISA technique with Alacramyn to determine serum and urine C. sculpturatus venom concentrations in envenomated patients.</p>
DOI10.1080/15563650802039965
Alternate JournalClin Toxicol (Phila)
PubMed ID18763156
Faculty Reference: 
Frank G. Walter, MD, FACEP, FACMT, FAACT
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