Retinal oximeter for the blue-green oximetry technique.

TitleRetinal oximeter for the blue-green oximetry technique.
Publication TypeJournal Article
Year of Publication2011
AuthorsDenninghoff KR, Sieluzycka KB, Hendryx JK, Ririe TJ, Deluca L, Chipman RA
JournalJ Biomed Opt
Date Published2011 Oct
ISSN Number1560-2281
KeywordsAdult, Animals, Humans, Light, Ophthalmoscopes, Optical Devices, Optical Phenomena, Oximetry, Oxygen, Oxyhemoglobins, Retinal Vessels, Scattering, Radiation, Sus scrofa

<p>Retinal oximetry offers potential for noninvasive assessment of central venous oxyhemoglobin saturation (SO(2)) via the retinal vessels but requires a calibrated accuracy of ±3% saturation in order to be clinically useful. Prior oximeter designs have been hampered by poor saturation calibration accuracy. We demonstrate that the blue-green oximetry (BGO) technique can provide accuracy within ±3% in swine when multiply scattered light from blood within a retinal vessel is isolated. A noninvasive on-axis scanning retinal oximeter (ROx-3) is constructed that generates a multiwavelength image in the range required for BGO. A field stop in the detection pathway is used in conjunction with an anticonfocal bisecting wire to remove specular vessel reflections and isolate multiply backscattered light from the blood column within a retinal vessel. This design is tested on an enucleated swine eye vessel and a retinal vein in a human volunteer with retinal SO(2) measurements of ∼1 and ∼65%, respectively. These saturations, calculated using the calibration line from earlier work, are internally consistent with a standard error of the mean of ±2% SO(2). The absolute measures are well within the expected saturation range for the site (-1 and 63%). This is the first demonstration of noninvasive on-axis BGO retinal oximetry.</p>

Alternate JournalJ Biomed Opt
PubMed ID22029366
PubMed Central IDPMC3206928
Grant List5T32EB000809-08 / EB / NIBIB NIH HHS / United States
Faculty Reference: 
Kurt Denninghoff, MD
Lawrence DeLuca, Jr., EdD, MD